Dynamic light scattering data interpretation

#DYNAMIC LIGHT SCATTERING DATA INTERPRETATION HOW TO#
#DYNAMIC LIGHT SCATTERING DATA INTERPRETATION FULL#

Graph these correlation values for a range of jumps of different durations and you get a Correlation Function (Figure 2). Most particles will be in totally different spots вЂ“ and you now have zero correlation in the data between your starting point and your jump one second later.

#DYNAMIC LIGHT SCATTERING DATA INTERPRETATION FULL#

Now instead of a microsecond, jump forward a full second. In other words, they have a high correlation. Odds are good most particles havenвЂ™t moved around yet вЂ“ so the light scattering hasnвЂ™t changed and the data from time zero and a microsecond later are about the same.

#DYNAMIC LIGHT SCATTERING DATA INTERPRETATION HOW TO#

HereвЂ™s how to think about analyzing light scattering data for DLS: pick a point in time вЂ“ now jump forward a microsecond. Analyzing whether light intensity is changing fast or slow вЂ“ thatвЂ™s the secret sauce of DLS. Vice versa for larger ones because they are slower to move around. Since small particles zip around quickly, the intensity of light changes quickly. When the laser wavelength is much larger than the particles, you get equal amounts of light scattering in every direction вЂ“ thatвЂ™s why we use a 660 nm laser in our DLS systems.ДLS can tell you a lot about the size of the particles in solution by measuring how rapidly that scattered light changes over time (Figure 1). Shine a laser on a solution of particles and youвЂ™ll get plenty of light scattering back out at you.

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Dynamic light scattering data interpretation